Biotechnology and its Application

1. Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?
A. ‘Flavr Savr’ tomato
B. Canolla
C. Golden rice
D. Bt-brinjal

2. RNA interferences is essential for the-
A. cell proliferation
B. cell defence
C. cell differentiation
D. micropropagation

3. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells)
A. both sense and anti-sense RNA
B. a particular hormone
C. an antifeedant
D. a toxic protein

4. Basic principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus of-
A. somatic cell
B. vegetative cell
C. germ cell
D. body cell

5. This is not a GMO.
A. Bt brinjal
B. Golden rice
C. Tracy
D. Dolly

6. Fearing that the child to be born may have a genetic disorder, a couple goes to a doctor. Which one of the following techniques is likely to be suggested by the doctor to cure the genetic disorder?
A. Hybridoma technology
B. Gene therapy
C. rDNA technology
D. Embryo transfer

7. Read the two statements A and B and identify the correct option from those given below.
Statement A : Agrobacterium tumefaciens is the causative agent of crown gall disease of dicots.
Statement B :Agrobacterium tumefaciens causes infection by entering the plant through wounds and injuries.
A. Statement A is correct and B is wrong.
B. Statement B is correct and A is wrong.
C. Both statement A and B are correct.
D. Both statements A and B are wrong.

8. Human proteins can be produced in the milk or semen of farm animals. True or false?
A. True
B. False, proteins cannot be produced in milk
C. False, proteins cannot be produced in semen
D. False, animals are not used for protein production

9. Which of the following is known as ‘Flavr Savr’?
A. Specific variety of pesticide
B. Breed of chicken
C. Transgenic tomato
D. Toxic insecticidal protein

10. Read the following four statements (A—D) about certain mistakes in two of them.
A. The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
B. Restrict1’on enzymes are used in isolation of DNA from other macromolecules.
C. Downstream processing is one of the steps of rDNA technology.
D. Disarmed pathogen vectors are also used in transfer of rDNA into the host.
Which of the two statements have mistakes?
A. B and C
B. C and D
C. A and C
D. A and B

11. Bacillus thuringiensis forms protein crystals which contain insectidical protein. This protein—
A. binds with epithelial cells of midgut of the insect pest ultimately killing it.
B. is coded by several genes including the gene cry.
C. is activated by acid pH of the foregut of the insect pest.
D. does not kill the carrier bacterium which is itself resistant to this toxin.

12. Select the wrong statement—
A. Human insulin is being commercially produced from a transgenic species of Escherichia coli.
B. The genetically modified Bacillus thuringiensis is used as biopesticide on the commercial scale.
C. Human protein, alpha-I antitrypsin is used to treat emphysema.
D. Bt toxin genes crylAc control the corn borer.

13. The proteins encoded by the genes crylAc and cryllAb control—
A. corn borer
B. budworms
C. butterflies
D. cotton bollworms

14. A transgenic food crop which may help in solving the problem of night blindness in developing countries is-
A. Starlink maize
B. Flavr Savr tomatoes
C. Golden rice
D. Bt soyabean

15. What is the advantage in clinical use of Humulin (human insulin produced through rDNA technique) over use of conventional ox or pig insulin?
A. It does not cause immunological problems
B. It is cheaper for the patient
C. There is no advantage
D. It is produced by E. coli in our own intestine.

16. The biological product created by the introduction of portions of DNA which codes for α-1 antitrypisin, is used to treat—
A. Asthma
B. Bronchitis
C. cystic fibrosis
D. emphysema

17. One of the advantages of developing transgenic mice is that it its very useful-
A. in producing new varieties of mice
B. to study vaccine safety
C. in developing a show piece example
D. in gene targeting

18. What is true of Bt toxin?
A. The concerned Bacillus has antitoxins.
B. Bt protein exists as active toxin in the Bacillus.
C. The inactive protein gets converted into active form in the insect gut.
D. Activate toxin enters ovaries of pest an sterilise them.

19. Which of the following genes do not occur naturally in living organism?
A. RNAi genes
B. Cry genes
C. Endogeneous cytoplasmic defense genes
D. Bt genes

20. The first human hormone produced by recombinant DNA technology is-
A. Insulin
B. Strogen
C. thyroxin
D. progesterone

Environmental Issues Excretory Products and their Elimination
Biodiversity and Conservation Body Fluids and Circulation
Ecosystem Breathing and Respiration
Organisms and Populations Digestion and Absorption
Biotechnology and its Application Plant Growth
Biotechnology Principles and Processes Respiration in Plants
Microbes in Human Welfare Photosynthesis
Strategies for Enhancement in Food Production Mineral Nutrition
Human Health and Disease Transport in Plants
Evolution Cell Cycle
Molecular Basic of Inheritance Biomolecules
Principle of Inheritance Cell the Unit of Life
Reproductive Health Structural Organisation
Human Reproduction Anatomy of Flowering Plants
Sexual Reproduction in Flowering Plants Morphology of Flowering Plants
Reproduction in Organisms Animal Kingdom
Chemical Coordination Plant Kingdom
Neural Control and Coordination Biological Classification
Locomotion and Movement The Living World

21. Insect pest resistant Bt cotton plant was developed using-
A. somaclonal variation
B. micropropagation
C. somatic hybridisation
D. transgenic technology

22. Ernst chain and Howrad Florey‘s contribution was-
A. discovery of streptokinase
B. establishing the potential of penicillin as an effective antibiotic
C. discovery of the DNA sequencer
D. isolating the bacterial plasmid.

23. RNA interference which is employed in making tobacco plant resistant to Meloidogyne incognita is essentially involved in-
A. preventing the process of replication of DNA
B. preventing the process of translation of mRNA
C. preventing the process of splicing of hnRNA
D. preventing the process of transcription

24. How many recombinant therapeutics have been used for human diseases throughout the world?
A. 12
B. 24
C. 30
D. 56

25. The strategy used to prevent the nematode infection in the roots of tobacco plant is called-
A. use of agro chemicals
B. Bt toxin gene
C. gene mutation
D. RNA interference

26. Gene therapy is a treatment that can be done with—
(i) adults only
(ii) Child or embryo only
(iii) pregnant mothers only
(iv) persons of any age and any condition.
A. (i) & (ii)
B. (ii) & (iii)
C. (iii) & (iv)
D. None of these

27. Bacillus thuringiensis (Bt) strains have been used for designing novel
a. bio-fertilisers
b. bio-insecticidal plants
c. bio-mineralisation process
d. bio-metallurgical techniques

28. Maximum number of existing transgenic animals is of—
a. Fish
b. Mice
c. cow
d. pig

29. Which one of the following bacteria is used for production of transgenic plants?
a. Escherichia coli
b. Bacillus thuringiensis
c. Staphylococcus aureus
d. Agrobactelum tumefaciens

30. In hybridoma technology-
A. B-cells are fused with myeloma cells
B. T-cells are fused with myeloma cells
C. B-cells are fused with T-cells
D. none of these above

31. During the processing of the prohormone “proinsulin” into the mature “insulin”—
A. C-peptide is added to proinsulin
B. C-peptide is removed from proinsulin
C. B-peptide is added to proinsulin
D. B-peptide is removed from proinsulin

32. Which one of the following is now being commercially produced by biotechnological procedure?
A. Nicotine
B. Morphine
C. Quinine
D. Insulin

33. The genetically-modified (GM) brinjal in India has been developed for—
A. enhancing mineral content
B. enhancing shelf life
C. insect-resistance
D. drought-resistance.

34. An improved variety of transgenic basmati rice—
A. does not require chemical fertilisers and growth hormones
B. gives high yield and is rich in vitamin A
C. is completely resistant to all insect pests and diseases of paddy
D. gives high yield but has no characteristic aroma.

35. Some of the characteristics of Bt cotton are—
A. long fibre and resistance to aphids
B. medium yield, long fibre and resistance to beetle pests
C. high yield and production of toxic protein crystals which kill dipteran pests.
D. high yield and resistance to bollworms.

36. Genetic engineering has been successfully used for producing—
A. transgenic mice for testing safety of polio vaccine before use in humans.
B. transgenic models for studying new treatments for certain cardiac diseases
C. transgenic cow-Rosie which produces high fat milk for making ghee
D. animals like bulls for farm work as they have super power.

37. First genetically modified plant commercially release in India is-
A. golden rice
B. slow ripening tomato
C. Bt-brinjal
D. Bt-cotton

38. Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.CoIi in bioreactors.
(iii) Purification of humulin.
(iv) introduction of recombinant plasmid into E. Coli.
(v) Extraction of recombinant gene product from E.CoIi
A. (ii), (i), (iv), (iii), (v), (vi)
B. (i), (iii), (v), (vi), (ii), (iv)
C. (i), (iv). (v), (ii). (vi), (iii)
D. (iii), (v), (ii), (i), (vi), (iv)

39. Salt tolerant transgenic has been developed for—
A. Brinjal
B. Grape
C. Potato
D. tomato

40. ‘Gray biotechnology’ is referred to—
A. medical process
B. industrial process
C. agricultural process
D. aquatic process

41. cryllAb and crylAb produce toxins that control—
A. cotton bollworms and corn borer respectively
B. corn borer and cotton bollworms respectively
C. tobacco budworms and nematodes respectively
D. corn borer and tobacco budworms respectively.

42. Which of the following is a transgenic plant?
A. Flavr Savr
B. Ashbya gosspii
C. Meloidogyne incognito
D. Gluconobacter oxidans

43. The illegal and unlawful development of biomaterials without payment to the inhabitants of their origin is called-
A. Biopatent
B. Biotechnology
C. Biowar
D. biopiracy

44. Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency?
A. Gene therapy
B. Chemotherapy
C. Immunotherapy
D. Radiation therapy

45. Which of the following statements about transgenic animals is/are false?
A. Transgenic animals are designed to study how genes are regulated
B. They are specially made to serve as models for human diseases
C. Transgenic cow Rosie was created to produce the human protein α-1 antitrypsin.
D. Transgenic mice are used to test the safety of vaccines
A. (iii) only
B. (i) and (iii) only
C. (ii) only
D. (ii) and (m) only

46. Desired genes have been introduced into transgenic animals to obtain large scale production of useful biological products encoded by these genes. This approach is generally referred to as-
A. Gene therapy
B. Hybridoma technology
C. Down stream processing
D. Molecular farming

47. Assertion : GM foods are facing widespread resistance by the people.
Reason : GM foods have mutated genes which cause infections and allergies.
A. If both assertion and reason are true and reason is the correct explanation of assertion.
B. If both assertion and reason are true but reason is not the correct explanation of assertion.
C. If assertion is true but reason is false.
D. If both assertion and reason are false.

48. An example of gene therapy is—
A. production of injectable hepatitis B vaccine
B. @producfion of vaccines in food crops like potatoes which can be eaten
C. introduction of gene for adenosine deaminase in person suffering from Severe Combined Immune Deficiency (SClD)
D. production of test tube babies by artificial insemination and implantation of fertilised eggs.

49. The introduction of T-DNA into plants involves
A. exposing the plants to cold for a brief period
B. allowing the plant roots to stand in water
C. altering the pH of the soil, then heat-shocking the plants
D. infection of the plant by Agrobacterium tumefaciens

50. Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of—
A. omega 3
B. vitamin A
C. vitamin B
D. vitamin C

51. Which one of the following statements is wrong in relation to transgenic Bt cotton plant?
A. Crop yield loss due to attack by lepidopteran insect pests is reduced.
B. The use of chemical insecticides in the cotton field is minimised.
C. Better quality cotton is produced
D. Crop yield loss due to attack by Bacillus thuringiensis bacterium is reduced.

52. A dicotyledonous plant forms crown gall when-
A. Agrobacterium tumefaciens comes in contact with plant
B. Agrobacterium rhizogenes comes in contact with the plant.
C. a specific part of DNA from the Ti plasmid gets integrated with the plant chromosome.
D. a specific part of DNA from the Ri plasmid gets integrated with the plant chromosome.

53. Which of the following Bt crops is being grown in India by the farmers?
A. Brinjal
B. Soyabean
C. Maize
D. Cotton

54. Genetically modified (GM) crops can be produced by-
A. recombinant DNA technology
B. somatic hybridisation
C. cross breeding
D. micropropagation

55. Which one of the following vectors are used to replace the defective gene in gene therapy?
A. Adenovirus
B. Cosmid
C. Ri plasmid
D. Ti plasmid